Background: The major histocompatibility complex (MHC) is the best-characterized genetic region related to resistance/\nsusceptibility to a wide range of infectious and immune-mediated diseases. Evidences suggest that MHC class II\ngenes may play an important role in developing different types of tumors including breast cancer. Canine mammary\ngland tumors (CMTs) are the most common neoplasms in female dogs. In the current study, the association of canine\nMHC class II DLA-DRB1.2 genotypes with development of mammary gland tumor profiles in dogs was investigated.\nDLA-DRB1.2 allelic diversity was determined in 40 dogs (18 CMT cases and 22 controls) using HRM technique and\nDNA sequencing. Association of the DLA-DRB1.2 genotypes with CMT profiles was expressed as odds ratio (OR).\nResults: Based on the histopathological typing of tumors, CMT cases were categorized into 4 groups: simple carcinoma,\ncomplex carcinoma, carcinoma arising in a benign tumor and special types of carcinoma. A total of eight HRM\nprofiles (A to H) were identified in dogs sampled. The association study revealed a significant correlation between\nDLA-DRB1.2 genotypes with different CMT profiles. The E genotype was significantly associated with increased risk of\ncarcinoma arising in a benign tumor, and the B genotype represented a positive correlation with complex carcinoma.\nSignificant association was also observed between the heterozygosity of DLA-DRB1.2 genotypes and decreased risk\nof developing tumor in dogs.\nConclusions: These results provide additional support for the association between DLA-DRB1 genes and development\nof mammary gland tumors in dogs and could potentially be used for early diagnosis of neoplasia and identifying\nsusceptible dogs.
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